ACROSOME DAMAGE AND ENZYME LEAKAGE OF GOAT SPERMATOZOA DURING DILUTION, COOLING AND FREEZING

Semen of Jamunapari goat bucks was frozen in three diluents egg yolk-t ris, egg yolk/citrate/glucose, and skim milk/egg yolk. In fresh ejacul ated semen over 90% of the spermatozoa had normal head and acrosome mo rphology. Quantification of goat sperm structure with Giemsa stain rev ealed significant (P < 0.01) damage to acrosome on freezing which vari ed between 38 to 43% in three diluents. Scanning electron microscopy d efined and revealed greater damage during freezing with 50% sperm head s having normal acrosome structure in three diluents. The ultrastructu ral changes detected in frozen goat sperm was protrusion at the anteri or cap, broken tail, swelling of acrosome, and loss of acrosomal conte nts. The leakage of five enzymes GOT, GPT, hyaluronoglucosaminidase, a cid phosphatase, and alkaline phosphatase measured simultaneously reve aled a positive correlation between enzyme release and acrosomal damag e.