The model described here involves a highly mechanistic view of anaerob
ic attached growth processes, considering a four-culture microbial pop
ulation interacting with and transforming six metabolic substrates in
two distinct reactor regions. These regions include 1) the biofilm zon
e, which is a thin layer consisting of highly concentrated bacterial m
ass embedded within a polysaccharide matrix; and 2) the bulk liquid re
gion, corresponding to the interstitial volume surrounding the biomass
support media. Mathematical descriptions of bacterial population dyna
mics and associated substrate transformation rates in both of these re
gions are presented. This model, because of its detailed description o
f fundamental processes in attached growth fermentation systems, provi
des an analytical tool for exploring both porous and solid media anaer
obic reactor systems.