SELECTION OF HUMAN ANTIHAPTEN ANTIBODIES FROM SEMISYNTHETIC LIBRARIES

Authors
BARBAS CF AMBERG W SIMONCSITS A JONES TM LERNER RA
Citation
Cf. Barbas et al., SELECTION OF HUMAN ANTIHAPTEN ANTIBODIES FROM SEMISYNTHETIC LIBRARIES, Gene, 137(1), 1993, pp. 57-62
Citations number
26
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
137
Issue
1
Year of publication
1993
Pages
57 - 62
Database
ISI
SICI code
0378-1119(1993)137:1<57:SOHAAF>2.0.ZU;2-1
Abstract
Semisynthetic human Fab libraries were constructed, displayed on the s urface of filamentous phage and selected for binding to three hapten c onjugates. A number of Fabs were isolated and characterized with respe ct to affinity and specificity. Fabs exhibited affinities of between 8 0 and 29 nM, as determined by surface plasmon resonance, for the conju gate on which they were selected. Conservation of Asp(101) in the thir d heavy-chain complementarity determining region (HCDR3) appears to be important in the construction of synthetically diverse repertoires.