FMOC SOLID-PHASE SYNTHESIS OF SERINE PHOSPHOPEPTIDES VIA SELECTIVE PROTECTION OF SERINE AND ON RESIN PHOSPHORYLATION

An Fmoc based solid phase method for the synthesis of phosphoserine pe ptides has been developed which is amenable to standard protocols on a utomated synthesizers. The serine residue to be phosphorylated is prot ected with a t-butyldimethylsilyl group which is selectively removed f rom the resin bound protected peptide. On resin phosphorylation and fi nal deprotection/cleavage are performed in standard fashion to yield s erine phosphopeptides of significant length and complexity.