PURIFICATION AND CHARACTERIZATION OF THE RESTRICTION-ENDONUCLEASE ITAI FROM ILYOBACTER-TARTARICUS RECOGNIZING 5'-GC-DOWN-ARROW-NGC-3'

Authors
STADTLER P VONSTRANDMANN RP WALTER T FREY B AUER H HENGSTENBERG W SCHMITZ G
Citation
P. Stadtler et al., PURIFICATION AND CHARACTERIZATION OF THE RESTRICTION-ENDONUCLEASE ITAI FROM ILYOBACTER-TARTARICUS RECOGNIZING 5'-GC-DOWN-ARROW-NGC-3', Gene, 137(2), 1993, pp. 347-348
Citations number
3
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
137
Issue
2
Year of publication
1993
Pages
347 - 348
Database
ISI
SICI code
0378-1119(1993)137:2<347:PACOTR>2.0.ZU;2-4
Abstract
ItaI, an isoschizomer of the subclass IIW [Kessler and Manta, Gene 92 (1990) 1-248] restriction endonuclease (ENase), Fnu4HI [Leung et al., Nucleic Acids Res. 6 (1979) 17-25], has been isolated from Ilyobactel tartaricus. The ENase has the five-base palindromic recognition sequen ce, 5'-GC down arrow NGC-3'. It cleaves behind the second nucleotide a nd produces a one-nt 5' overhang.