EQUIPMENT FOR THE LARGE-SCALE ELECTROMANIPULATION OF PLANT-PROTOPLASTS

Authors
JONES B LYNCH PT HANDLEY GJ MALAURE RS BLACKHALL NW HAMMATT N POWER JB COCKING EC DAVEY MR
Citation
B. Jones et al., EQUIPMENT FOR THE LARGE-SCALE ELECTROMANIPULATION OF PLANT-PROTOPLASTS, BioTechniques, 16(2), 1994, pp. 312-321
Citations number
30
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
16
Issue
2
Year of publication
1994
Pages
312 - 321
Database
ISI
SICI code
0736-6205(1994)16:2<312:EFTLEO>2.0.ZU;2-Q
Abstract
Electric fields are now used extensively for the genetic manipulation of plant cells through protoplast fusion and direct gene uptake. The c ost of commercially available electrofusion and electroporation equipm ent remains prohibitive for many laboratories. This paper describes an electronic apparatus, suitable for the large-scale electrofusion and electroporation of plant protoplasts that is compatible in both functi on and cost with commercially available equipment.