The effect of cuticular lipids of Ostrinia nubilalis (Hubner) and Melo
lontha melolontha (L.) spore germination and hyphal growth of entomopa
thogenous fungi was examined. A Beauveria bassiana strain virulent to
O. nubilalis and a Beauveria brogniartii strain virulent to ill. melol
ontha were used. The epicuticular lipid-extract of the host insect did
not alter the growth of the corresponding virulent strain measured bo
th as spore germination or hyphal growth. However, the cuticular penta
ne-extract of M. melolontha inhibited both the hyphal growth and spore
germination of the nonpathogenic strain of B. bassiana. The hyphal in
hibition was caused mostly by the unsaturated hydrocarbon fraction. Me
thanol-extracts of both insects inhibited hyphal growth and spore germ
ination of the 2 virulent and nonvirulent strains. Exposure to O. nubi
lalis lipid extract most strongly inhibited hyphal growth of both stra
ins.