EFFECT OF CONDITIONS OF HYPOTHERMIA ON SYNTHESIS OF STRESS PROTEINS IN WINTER-WHEAT SEEDLINGS

We used the radioisotope method, two-dimensional electrophoresis, and fluorography to study synthesis of stress proteins in etiolated shoots of winter wheat (Triticum aestivum L.) under different conditions of hypothermia. It is demonstrated that short-term temperature stress ind uces synthesis of a number of cold and heat shock proteins. Short-term reiterated action of hypothermia leads to increase in content of a nu mber of stress proteins (15, 32, 33, 55, and 150 kD). Under these cond itions, frost resistance of the seedlings increases parallel with synt hesis of stress proteins. It is hypothesized that a trigger mechanism for induction of synthesis of a number of stress proteins functions in plants.