SELECTION AND MICROINJECTION OF ACROSOME-REACTED HUMAN SPERMATOZOA

A method of selection of acrosome-reacted human spermatozoa is describ ed. Petri dishes were coated with GB24 antibody, specific to the inner acrosomal membrane. The acrosome-reacted spermatozoa were fixed on th e antibody and could be removed by aspiration with a micro-pipette. Th ey were then injected into the perivitelline space of hamster eggs in order to check their fertilizing ability. This selection allowed the f ertilization rate to be significantly increased (24 versus 7% for cont rol spermatozoa; P < 0.01). This method could enhance the results of h uman sub-zonal insemination.