G. Chiocchia et Ka. Smith, HIGHLY SENSITIVE METHOD TO DETECT MESSENGER-RNAS IN INDIVIDUAL CELLS BY DIRECT RT-PCR USING TTH DNA-POLYMERASE, BioTechniques, 22(2), 1997, pp. 312
A new method for detecting the expression of low-abundance mRNA molecu
les has been developed that combines the sensitivity of PCR, the high
efficiency and specificity of reverse transcription (RT) using Tth DNA
polymerase at high temperature, and the enhancement of sensitivity an
d specificity of nested PCR. This method is highly sensitive, reproduc
ible and allows the detection of MRNAs in individual cells by direct R
T-PCR.