EFFECT OF ALUMINUM AND LOW PH ON THE CALL US INDUCTION AND GREEN PLANT-REGENERATION IN ANOTHER CULTURE

Due to the acidification of the soils the issue of aluminium toxicity will be more and more emphasized. A part of the varieties grown in Hun gary possesses, however, a certain degree of tolerance, but it deemes to be advisable to increase this tolerance and to widen the group of t olerant genotypes. This may be achieved in the simpliest way by applyi ng haploid mutant selection methods. But in order to develop an effect ive method, it is indispensable to know how the aluminium and low pH g iven to the induction nutrient medium would influence the anther induc tion and the fertile green plant regeneration. Based on the authors' r esults, the aluminium toxicity can be well simulated also in anther cu lture, the 50 muM1-1 solution of Al3+ applied to the nutrient medium w ith low pH (pH: 4.2 and 4.5) reduces considerably the callus induction . The higher concentrations of Al3+ (100 and 250 muM1-1) destroy entir ely the microspore population. On regeneration nutrient medium 190-2 w ith normal pH, the calluses originating from induction nutrient medium containing aluminium may be regenerated by adequate frequency. Accord ing to the seedling test results of grains originating from fertile di haploid plants, an increase may be expected in the variability of alum inium tolerance even if the aluminium treatment is applied only in the induction phase. The multiplication of progenies and the testing of t heir aluminium tolerance are now going on.