CLONING OF EGGPLANT HYPOCOTYL CDNAS ENCODING CYTOCHROMES P450 BELONGING TO A NOVEL FAMILY (CYP77)

Authors
TOGURI T TOKUGAWA K
Citation
T. Toguri et K. Tokugawa, CLONING OF EGGPLANT HYPOCOTYL CDNAS ENCODING CYTOCHROMES P450 BELONGING TO A NOVEL FAMILY (CYP77), FEBS letters, 338(3), 1994, pp. 290-294
Citations number
26
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
338
Issue
3
Year of publication
1994
Pages
290 - 294
Database
ISI
SICI code
0014-5793(1994)338:3<290:COEHCE>2.0.ZU;2-E
Abstract
From eggplant hypocotyl tissues we have cloned two closely related cDN As encoding cytochromes P450 (P450s) by PCR amplification using a prim er designed based on the highly conserved sequence among the known egg plant P450s. One cDNA lacks the NH2-terminal short sequence that is pr esent in the other, full-length cDNA. The two predicted protein sequen ces are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which ar e termed CYP77A1 and -A2, belong to a hitherto unknown P450 family.