KINETICS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE TRANSCRIPTIONIN BLOOD MONONUCLEAR PHAGOCYTES ARE SLOWED BY LIMITATIONS OF NUCLEOTIDE PRECURSORS
Wa. Obrien et al., KINETICS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE TRANSCRIPTIONIN BLOOD MONONUCLEAR PHAGOCYTES ARE SLOWED BY LIMITATIONS OF NUCLEOTIDE PRECURSORS, Journal of virology, 68(2), 1994, pp. 1258-1263
Human immunodeficiency virus type 1 infection of mononuclear phagocyte
s has been implicated in disease manifestations, but postentry viral r
eplication events in these cells have not been well characterized. Pro
ductive infection of activated T cells is associated with cell prolife
ration and accumulation of full-length viral DNA within 6 h. In infect
ed, nondividing quiescent peripheral blood lymphocytes, reverse transc
ription is aborted prior to full-length viral DNA formation. For nondi
viding, cultured mononuclear phagocytes, we now report a third pattern
of reverse transcription with relatively slow kinetics, in which full
-length viral DNA did not accumulate until 36 to 48 h. The reverse tra
nscription rate in mononuclear phagocytes could be accelerated by addi
tion of exogenous nucleotide precursors, but still not to the rate see
n in activated T cells. These results indicate that substrate limitati
ons in mononuclear phagocytes slow but do not arrest human immunodefic
iency virus type 1 reverse transcription.