THE MOUSE COL11A2 GENE - SOME TRANSCRIPTS FROM THE ADJACENT RXR-BETA GENE EXTEND INTO THE COL11A2 GENE

Type XI collagen is present in small amounts in cartilage, together wi th small amounts of type IX and type V collagens and large amounts of type II collagen. Here, primers based on the nucleotide sequences of p artial human cDNAs and mouse genomic DNAs that were analyzed by other investigators were used to isolate a cDNA for the mouse col11a2 gene. Cosmid clones for the mouse col11a2 gene were isolated, and 12.4 kb of the nucleotide sequences were defined. Analysis of the genomic sequen ces identified three exons in the mouse gene that were recently shown to undergo alternative splicing (Tsumaki and Kimura, J. Biol. Chem. 27 0, 2372-2378, 1995; Zhidkova et al., J. Biol. Chem. 270, 94886-9493, 1 995). In addition, analysis of the cosmid clones revealed that the 5' end of the mouse col11a2 gene was located head-to-tail with the mouse retinoic X receptor beta gene. RT-PCR assays demonstrated that some tr anscripts from the retinoic X receptor beta gene extend into the col11 a2 gene. Therefore, there may be coordinate expression of the two gene s.