ISOLATION AND CHARACTERIZATION OF AN N-ACETYL-D-GALACTOSAMINE-BINDINGLECTIN FROM DUTCH IRIS BULBS WHICH RECOGNIZES THE BLOOD-GROUP-A DISACCHARIDE (GALNAC-ALPHA-1-3GAL)

Authors
MO HQ VANDAMME EJM PEUMANS WJ GOLDSTEIN IJ
Citation
Hq. Mo et al., ISOLATION AND CHARACTERIZATION OF AN N-ACETYL-D-GALACTOSAMINE-BINDINGLECTIN FROM DUTCH IRIS BULBS WHICH RECOGNIZES THE BLOOD-GROUP-A DISACCHARIDE (GALNAC-ALPHA-1-3GAL), The Journal of biological chemistry, 269(10), 1994, pp. 7666-7673
Citations number
33
Categorie Soggetti
Biology
Journal title
The Journal of biological chemistry → ACNP
ISSN journal
00219258
Volume
269
Issue
10
Year of publication
1994
Pages
7666 - 7673
Database
ISI
SICI code
0021-9258(1994)269:10<7666:IACOAN>2.0.ZU;2-C
Abstract
A blood group type A disaccharide (GalNAc alpha 1-3Gal)binding lectin has been purified from Dutch Iris (Iris x hollandica) bulbs to electro phoretic homogeneity by consecutive affinity chromatography on columns of immobilized asialofetuin-Sepharose 4B and Synsorb A disaccharide ( GalNAc alpha 1-3Gal beta-O-(CH2)(8)CONH-Synsorb). This lectin agglutin ates both native and trypsin-treated rabbit erythrocytes, but not huma n erythrocytes, irrespective of blood group type.