MONOCLONAL-ANTIBODIES IN BLOOD-GROUP SERO LOGY .1. TECHNIQUE OF PRODUCTION, ABO AND LEWIS SYSTEM

Objective: The introduction of monoclonal antibodies in routine blood group typing has also given rise to new findings. This paper was desig ned to demonstrate and clarify modifications relevant in practice. Sou rces: Our own experience in the production and application of monoclon al antibodies as well as the relevant literature are taken into consid eration. Results: The development and production of murine and human m onoclonal antibodies is described especially regarding the selection a nd the safety of clones. An update of the availability and use of mono clonal antibodies in respect to the different blood group systems is g iven, with special reference to new findings like the A(1)(B) and B(A) phenomena. Conclusion: The application of monoclonal antibodies in bl ood group serology has improved the detection of weak variants and the availability of these reagents in general. Further improvements in th is technology will make monoclonal antibodies also available for other blood group systems.