PRODUCTION OF DOMINANT FEMALE STERILITY IN DROSOPHILA-MELANOGASTER BYINSERTION OF THE OVO(D1) ALLELE ON AUTOSOMES - USE OF TRANSFORMED STRAINS TO GENERATE GERMLINE MOSAICS
M. Mevelninio et al., PRODUCTION OF DOMINANT FEMALE STERILITY IN DROSOPHILA-MELANOGASTER BYINSERTION OF THE OVO(D1) ALLELE ON AUTOSOMES - USE OF TRANSFORMED STRAINS TO GENERATE GERMLINE MOSAICS, Mechanisms of development, 45(2), 1994, pp. 155-162
We have cloned a 7 kb genomic fragment containing the dominant female-
sterile mutation ovo(D1). This fragment confers to transgenic females
a sterility phenotype, the severity of which depends both on the genet
ic background and the ratio of ovo(D1) product to ovo(+) product. Fema
les containing two copies of the ovo(D1) transgene, or those containin
g one recessive null allele at the ovo locus, are about as sterile as
ovo(D1) females. Twenty transformed strains were obtained and five of
them were tested and shown to be excellent tools for identifying a ger
mline clone of cells sustaining mitotic recombination on the autosomes
. One of the tested strains carries an insert on chromosome 4, which e
nabled us to show that mitotic recombination on that chromosome is not
a rare event: it is in fact frequent enough for the maternal effects
of the zygotic lethal mutations cubitus interruptus Dominant (ci(D)) a
nd l(4)29 to be studied.