RETENTION OF THE HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) UL37 PROTEIN ON SINGLE-STRANDED-DNA COLUMNS REQUIRES THE HSV-1 ICP8 PROTEIN

The UL37 and ICP8 proteins present in herpes simplex virus type 1 (HSV -1)-infected-cell extracts produced at 24 h postinfection coeluted fro m single-stranded-DNA-cellulose columns. Experiments carried out with the UL37 protein expressed by a vaccinia virus recombinant (V37) revea led that the UL37 protein did not exhibit DNA-binding activity in the absence of other HSV proteins. Analysis of extracts derived from cells coinfected with V37 and an ICP8-expressing vaccinia virus recombinant (V8) and analysis of extracts prepared from cells infected with the H SV-1 ICP8 deletion mutants d21 and n10 revealed that the retention of the UL37 protein on single-stranded DNA columns required a DNA-binding -competent ICP8 protein.