HPLC AND NMR INVESTIGATION OF THE SERUM AMINE OXIDASE CATALYZED OXIDATION OF POLYAMINES

In the presence of amine oxidases polyamines arrest cell proliferation owing to the generation of hydrogen peroxide and amino aldehydes. In this investigation the bovine serum amine oxidase catalyzed oxidation of polyamines has been studied by HPLC analysis of dansylated reaction products with or without NaBH4 reduction and by NMR spectroscopy of t he reaction products and 3-aminopropanal was found to be a major react ion product. These findings were further substantiated by analysis of the reaction products by ion-exchange chromatography and by analysis o f the products formed by oxidation of polyamines by the cofactor of Cu amine oxidases, 6-hydroxydopa. 3-Aminopropanal is unstable and can gi ve rise to acrolein by beta-elimination.