E. Sivvas et al., SYNTHESIS OF A NEW KAINIC ACID-BASED SELECTIVE LIGAND AS A POTENTIAL PHOTOAFFINITY LABEL OF NON-NMDA EXCITATORY AMINO-ACID RECEPTORS IN CHICKEN BRAIN, Acta chemica Scandinavica, 48(1), 1994, pp. 76-79
The synthesis of two analogues of kainic acid (KA) incorporating photo
-activatible moieties attached either on the gamma-carboxy function (g
amma-amide 1) or the isopropenyl side-chain (amide 2) is described. Th
e synthesis of the former amide involves coupling of N-(tert-butoxycar
bonyl)-protected alpha-diphenylmethyl kainate with 2-(4-azidobenzamido
)ethylamine (5) followed by trifluoroacetic acid mediated complete dep
rotection. Amide 2 was synthesized by palladium-mediated allylic amina
tion, with 4,4'-dimethoxybenzhydrylamine (DMBA), of N-(9-fluorenylmeth
oxycarbonyl)-protected dimethyl kainate, followed by splitting the DMB
-group with formic acid, coupling with N-hydroxysuccinimidoyl 4-azidob
enzoate and finally complete deprotection by saponification. Prelimina
ry pharmacological studies in chicken brain membranes showed that amid
e 2 is a stronger inhibitor of [H-3]KA binding on chicken cerebellar m
embranes than is amide I and that amide 2 has specificity only for the
cerebellar, as opposed to the telencephalon, type of non-NMDA binding
sites.