HUMAN TONSIL, BLOOD AND BONE-MARROW IN-VIVO INDUCED B-CELLS CAPABLE OF SPONTANEOUS AND HIGH-RATE IMMUNOGLOBULIN SECRETION IN-VITRO - DIFFERENCES IN THE REQUIREMENTS FOR FACTORS AND FOR ADHERENT AND BONE-MARROWSTROMAL CELLS, AS WELL AS DISTINCTIVE ADHESION MOLECULE EXPRESSION
Ja. Brieva et al., HUMAN TONSIL, BLOOD AND BONE-MARROW IN-VIVO INDUCED B-CELLS CAPABLE OF SPONTANEOUS AND HIGH-RATE IMMUNOGLOBULIN SECRETION IN-VITRO - DIFFERENCES IN THE REQUIREMENTS FOR FACTORS AND FOR ADHERENT AND BONE-MARROWSTROMAL CELLS, AS WELL AS DISTINCTIVE ADHESION MOLECULE EXPRESSION, European Journal of Immunology, 24(2), 1994, pp. 362-366
Human B cells capable of spontaneous IgG secretion are commonly found
in circulation and in lymphoid tissues such as tonsil and bone marrow
(BM). The present study compares the mechanisms that regulate tonsil,
blood and BM B cells capable of spontaneous IgG secretion, The BM cell
subset produced IgG during a markedly longer period of time (14 days)
than did tonsil and blood cell subsets (2-3 days). Blood and BM, but
not tonsil, B cell IgG secretion depended on the presence of adherent
cells, as demonstrated by adherent cell depletion and re-addition expe
riments. Stromal BM cells supported linear IgG secretion by non-adhere
nt BM cells for 2 weeks, but were unable to prolong the short-term IgG
secretion by tonsil and blood cells. Different factors induced IgG se
cretion in each of the three B cell populations as optimal IgG secreti
on by tonsil, blood or BM cell subsets required either tumor necrosis
factor-alpha, interleukin-6 or fibronectin + interleukin-6, respective
ly. Finally, these populations also showed differences in the expressi
on of adhesion molecules; the tonsilar cell subset was PNA(+/-) CD44() CD19d(+) CD19e(-) Leu-8(+/-), the blood cell subset was PNA(-) CD44(
+/-) CD49d(+) CD49e(-) Leu-8(+) and the BM cell subset was PNA(-) CD44
(+/-) CD49d(+) CD49e(-) Leu-8(-). These results suggest that the mecha
nisms controlling the final differentiation and the expression of adhe
sion molecules in these B lymphocytes exhibit territorial specificity.