Aa. Vertes et al., ISOLATION AND CHARACTERIZATION OF IS31831, A TRANSPOSABLE ELEMENT FROM CORYNEBACTERIUM-GLUTAMICUM, Molecular microbiology, 11(4), 1994, pp. 739-746
A transposable element from a coryneform bacterium, Corynebacterium gl
utamicum ATCC 31831 was isolated and characterized. The element IS3183
1 is a 1453 bp insertion sequence with 24 bp imperfect terminal invert
ed repeats. It contains one open reading frame highly homologous at th
e amino acid level to the transposase of IS1096 from Mycobacterium sme
gmatis. Both IS31831 and IS1096 exhibit several common characteristics
suggesting that they constitute a new family of insertion sequences.
IS31831 was isolated by taking advantage of the sucrose sensitivity of
coryneform bacteria conferred by expression of the Bacillus subtilis
sacB gene. An Escherichia coli/Corynebacterium shuttle vector useful f
or the isolation of transposable elements from the coryneform group of
bacteria was constructed.