ENZYME-LINKED-IMMUNOSORBENT-ASSAY ENZYME-LINKED COAGULATION ASSAY FORDETECTION OF ANTIBODIES TO CLOSTRIDIUM-BOTULINUM NEUROTOXIN-A, NEUROTOXIN-B, AND NEUROTOXIN-E AND SOLUTION-PHASE COMPLEXES

Authors
DOELLGAST GJ TRISCOTT MX BEARD GA BOTTOMS JD
Citation
Gj. Doellgast et al., ENZYME-LINKED-IMMUNOSORBENT-ASSAY ENZYME-LINKED COAGULATION ASSAY FORDETECTION OF ANTIBODIES TO CLOSTRIDIUM-BOTULINUM NEUROTOXIN-A, NEUROTOXIN-B, AND NEUROTOXIN-E AND SOLUTION-PHASE COMPLEXES, Journal of clinical microbiology, 32(3), 1994, pp. 851-853
Citations number
5
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
32
Issue
3
Year of publication
1994
Pages
851 - 853
Database
ISI
SICI code
0095-1137(1994)32:3<851:EECAF>2.0.ZU;2-K
Abstract
The solution-phase complex assay for toxins A, B, and E from Clostridi um botulinum (Elcatech, Inc., Winston-Salem, N.C.) was modified to mea sure antibody. The addition of unlabeled polyclonal antibodies to a mi xture consisting of toxin with chicken antibody and RVV-XA-labeled hor se antibody reduces the sensitivity of detection of neurotoxin. This r eduction in sensitivity can be used as a measure of the specific antib ody titer.