ENZYME-LINKED-IMMUNOSORBENT-ASSAY ENZYME-LINKED COAGULATION ASSAY FORDETECTION OF ANTIBODIES TO CLOSTRIDIUM-BOTULINUM NEUROTOXIN-A, NEUROTOXIN-B, AND NEUROTOXIN-E AND SOLUTION-PHASE COMPLEXES
Gj. Doellgast et al., ENZYME-LINKED-IMMUNOSORBENT-ASSAY ENZYME-LINKED COAGULATION ASSAY FORDETECTION OF ANTIBODIES TO CLOSTRIDIUM-BOTULINUM NEUROTOXIN-A, NEUROTOXIN-B, AND NEUROTOXIN-E AND SOLUTION-PHASE COMPLEXES, Journal of clinical microbiology, 32(3), 1994, pp. 851-853
The solution-phase complex assay for toxins A, B, and E from Clostridi
um botulinum (Elcatech, Inc., Winston-Salem, N.C.) was modified to mea
sure antibody. The addition of unlabeled polyclonal antibodies to a mi
xture consisting of toxin with chicken antibody and RVV-XA-labeled hor
se antibody reduces the sensitivity of detection of neurotoxin. This r
eduction in sensitivity can be used as a measure of the specific antib
ody titer.