INFLUENCE OF CULTURE MEDIA ON PROSTAGLANDIN OUTPUT BY DISPERSED AMNION CELLS

The purpose of the study was to examine the influence of culture media on prostaglandin (PG) production by amnion cells and their response t o phospholipase A(2). Cells were dispersed from term tissue obtained b y elective C-section; the PGE(2) output was studied during the first 2 4 h of culture. The basal PGE(2) production from cells cultured in Med ia 199 (M199) supplemented by 10% horse serum (HS) was significantly g reater than that of cells cultured in F12:DME supplemented by 10% feta l calf serum (FCS). Furthermore, phospholipase A(2) stimulated PGE(2) production in cells cultured with M199 + HS but had no influence on PG E, output by cells cultured with F12:DME + FCS. This effect was depend ent on the presence of HS. The factor(s) in HS responsible was not rem oved by heating at 56 degrees C for 3 min, treatment with dextran coat ed charcoal or by ultrafiltration through 10 000 MW filters. Thus, cul ture media alters the in vitro production of PG and response to phosph olipase A(2) by amnion cells.