EVIDENCE FOR INVOLVEMENT OF PROTEIN-KINASE-C IN EXPRESSION OF INTRACELLULAR ADHESION MOLECULE-1 (ICAM-1) BY HUMAN VASCULAR ENDOTHELIAL-CELLS

Potent activators of protein kinase C (PKC), such as phorbol dibutyrat e and octylindolactam V, stimulated expression of intercellular adhesi on molecule 1 (ICAM-1) by human umbilical vein endothelial cells (HUVE C) in a concentration-dependent manner. Expression of PKC activator-in duced ICAM-1 in HUVEC was inhibited by the PKC inhibitor, H-7. Further more, cytokine (TNF alpha, LPS)-induced ICAM-1 expression was inhibite d by the potent PKC inhibitor, H-7, and not by the cAMP-dependent prot ein kinase (PKA) specific inhibitor, H-89. These data suggest that PKC is involved in cytokine- and inflammatory agent-induced upregulation of ICAM-1 expression in HUVEC.