DEGRADATION OF A POLYMERASE CHAIN-REACTION (PCR) PRODUCT BY HEAT-STABLE DEOXYRIBONUCLEASE (DNASE) PRODUCED FROM YERSINIA-ENTEROCOLITICA

Authors
NAKAJIMA H ITOH K ARAKAWA E INOUE M MORI T WATANABE H
Citation
H. Nakajima et al., DEGRADATION OF A POLYMERASE CHAIN-REACTION (PCR) PRODUCT BY HEAT-STABLE DEOXYRIBONUCLEASE (DNASE) PRODUCED FROM YERSINIA-ENTEROCOLITICA, Microbiology and immunology, 38(2), 1994, pp. 153-156
Citations number
7
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
38
Issue
2
Year of publication
1994
Pages
153 - 156
Database
ISI
SICI code
0385-5600(1994)38:2<153:DOAPC(>2.0.ZU;2-5
Abstract
When crude deoxyribonucleic acid (DNA) preparations by boiling were us ed for the polymerase chain reaction (PCR) from pathogenic and non-pat hogenic Yersinia enterocolitica strains, the amplified products were d egraded after their storage at 4 C. The degradation of products was pr evented by the addition of ethylenediaminetetraacetate (EDTA) or treat ment with proteinase K. These findings indicate that Y. enterocolitica produced heat-stable deoxyribonuclease (DNase). Proteinase K treatmen t would be recommended to prevent heat-stable DNase contamination in t he DNA preparations for PCR from Y. enterocolitica strains.