H. Nakajima et al., DEGRADATION OF A POLYMERASE CHAIN-REACTION (PCR) PRODUCT BY HEAT-STABLE DEOXYRIBONUCLEASE (DNASE) PRODUCED FROM YERSINIA-ENTEROCOLITICA, Microbiology and immunology, 38(2), 1994, pp. 153-156
When crude deoxyribonucleic acid (DNA) preparations by boiling were us
ed for the polymerase chain reaction (PCR) from pathogenic and non-pat
hogenic Yersinia enterocolitica strains, the amplified products were d
egraded after their storage at 4 C. The degradation of products was pr
evented by the addition of ethylenediaminetetraacetate (EDTA) or treat
ment with proteinase K. These findings indicate that Y. enterocolitica
produced heat-stable deoxyribonuclease (DNase). Proteinase K treatmen
t would be recommended to prevent heat-stable DNase contamination in t
he DNA preparations for PCR from Y. enterocolitica strains.