A denitrifying consortium capable of transforming carbon tetrachloride
(CCl4) was cultured from aquifer sediment from the U.S. Department of
Energy's Hanford Site in southeastern Washington State. To understand
the kinetics of the biological destruction of CCl4 by these microbes,
a set of experiments, the conditions of which were chosen according t
o a fractional factorial experimental design, were completed. This art
icle reports on the experimental design along with the results for CCl
4, biomass, acetate, nitrate, and nitrite concentrations. These data i
ndicate that growth is inhibited by high nitrite concentrations, where
as CCl4 degradation is slowed by the presence of nitrate and/or nitrit
e. (C) 1994 John Wiley and Sons, Inc.