PHARMACOKINETICS AND TISSUE DISTRIBUTION OF RECOMBINANT HUMAN TRANSFORMING GROWTH-FACTOR BETA(1) AFTER TOPICAL AND INTRAVENOUS ADMINISTRATION IN MALE-RATS
Tf. Zioncheck et al., PHARMACOKINETICS AND TISSUE DISTRIBUTION OF RECOMBINANT HUMAN TRANSFORMING GROWTH-FACTOR BETA(1) AFTER TOPICAL AND INTRAVENOUS ADMINISTRATION IN MALE-RATS, Pharmaceutical research, 11(2), 1994, pp. 213-220
Recombinant human transforming growth factor beta (rhTGF-beta(1)) enha
nces the healing process after topical application to various animal w
ound models. A detailed pharmacokinetic and tissue distribution study
was performed to support the clinical development of rhTGF-beta(1) for
wound healing indications. Rats received radioiodinated or unlabeled
rhTGF-beta(1) as an intravenous (iv) bolus or as a topical formulation
applied to a full thickness wound. Plasma concentrations of TGF-beta(
1) were estimated from TCA-precipitable radioactivity or were measured
by ELISA. Following iv administration, the initial half-life was rapi
d (<11 min), regardless of whether radiolabeled or unlabeled rhTGF-bet
a(1) was used. The terminal half-life was long (163 min) when the test
material was radioiodinated and administered as a trace dose and rela
tively short (less than or equal to 61 min) when given at high doses a
nd assayed by ELISA. Analysis of plasma radioactivity by SDS-PAGE reve
aled a time-dependent clearance of the 25-kDa parent molecule without
a significant appearance of lower molecular weight radiolabeled metabo
lites. The majority of the radioactivity was associated with highly pe
rfused organs, known iodide elimination pathways, and the thyroid at 1
and 8 hr after iv injection. After topical administration of a high d
ose (0.8 mg/kg), no immunoreactive TGF-beta(1) was detectable in plasm
a samples taken over a 48-hr period. However, trace amounts (less than
or equal to 0.05 ng/mL) of acid-precipitable radioactivity were detec
ted in plasma after topical application of [I-125]rhTGF-beta(1) (1 mu
g/kg, 126 mu Ci/kg). A significant portion (35%) of the [I-125]rhTGF-b
eta(1) persisted intact (25 kDa) at the wound site 24 hr after applica
tion. In conclusion, rhTGF-beta(1) was rapidly cleared after iv bolus
administration and distributed primarily to the liver, lungs, kidney,
and spleen. Little systemic exposure was observed after applying a sin
gle topical dose of rhTGF-beta(1) to a wound, and the intact molecule
persisted at the wound site.