EN-BLOC INCORPORATION OF COATOMER SUBUNITS DURING THE ASSEMBLY OF COP-COATED VESICLES

Authors
HARAKUGE S KUGE O ORCI L AMHERDT M RAVAZZOLA M WIELAND FT ROTHMAN JE
Citation
S. Harakuge et al., EN-BLOC INCORPORATION OF COATOMER SUBUNITS DURING THE ASSEMBLY OF COP-COATED VESICLES, The Journal of cell biology, 124(6), 1994, pp. 883-892
Citations number
28
Categorie Soggetti
Cytology & Histology
Journal title
The Journal of cell biology → ACNP
ISSN journal
00219525
Volume
124
Issue
6
Year of publication
1994
Pages
883 - 892
Database
ISI
SICI code
0021-9525(1994)124:6<883:EIOCSD>2.0.ZU;2-P
Abstract
The cDNA encoding epsilon-COP, the 36-kD subunit of coatomer, was clon ed from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-epsilon-COP antibody showed that epsilon-COP exists in COP-co ated vesicles as well as in the cytosolic coatomer. Using the cloned c DNA, recombinant His(6)-tagged epsilon-COP was overexpressed in cultur ed Chinese hamster ovary (CHO) cells, from which metabolically radiola beled coatomer was purified by taking advantage of the His(6) tag. Rad iolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.