SOLUTION STRUCTURE OF PYOVERDIN GM-II

The three-dimensional structure in solution of ferri-pyoverdin GM-II i solated from the culture medium of Pseudomonas fluorescens was determi ned by application of NMR methods to the Ga3+ analogue. Distance geome try calculations were performed with FILMAN using interproton distance s and coupling constants as constraints. Further conformational analys is was carried out by energy minimization with MM2 and AMBER. Back-cal culation of the NOESY spectra shows that the resulting structures are in agreement with the experimental data.