AN IMPROVED MTT ASSAY USING THE ELECTRON-COUPLING AGENT MENADIONE

Authors
GARN H KRAUSE H ENZMANN V DROSSLER K
Citation
H. Garn et al., AN IMPROVED MTT ASSAY USING THE ELECTRON-COUPLING AGENT MENADIONE, Journal of immunological methods, 168(2), 1994, pp. 253-256
Citations number
5
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
168
Issue
2
Year of publication
1994
Pages
253 - 256
Database
ISI
SICI code
0022-1759(1994)168:2<253:AIMAUT>2.0.ZU;2-D
Abstract
A modification of the MTT based tetrazolium colorimetric assay is desc ribed. Using the electron-coupling agent menadione formazan formation by murine splenocytes and P-815 cells was significantly increased wher eas dye reduction by macrophages was hardly influenced. These observat ions suggest that it should be possible to improve the tetrazolium bas ed cytotoxicity assays of murine macrophages against cells of the syng eneic tumour cell line P-815.