PHYSICAL MAP OF THE GENOME OF ACHOLEPLASMA-OCULI ISM1499 AND CONSTRUCTION OF A TN4001 DERIVATIVE FOR MACRORESTRICTION CHROMOSOMAL MAPPING

A physical chromosomal map of Acholeplasma oculi ISMI-499 was construc ted by using field inversion gel electrophoresis. To assist in the ord ering of the chromosomal fragments, a modified transposon, Tn4001.1064 , was constructed. It was also used to rescue mycoplasmal chromosomal sequences adjacent to transposon insertion sites in a one-step cloning procedure. The total size of the A. oculi ISM1499 genome was estimate d to be 1,633 kb. The restriction enzyme sites for ApaI, BssHII, EagI, and SmaI were positioned on the map along with several transposon ins ertion sites.