The nucleotide sequence of repeated sequence I, which appears in five
regions on nylon oligomer-degrading plasmid pOAD2, harbored in Flavoba
cterium sp. strain KI72, was determined. The five regions of repeated
sequence I had 880 bp of identical sequence, and the sequence was iden
tical to that of IS6100, an insertion sequence classified in the IS6 f
amily, initially found in Mycobacterium fortuitum. Sequences homologou
s to that of ZS6100 R ere found for another nylon oligomer-degrading p
lasmid, pNAD2, harbored in Pseudormonas sp. strain NK87, by Southern h
ybridization experiments.