GENERATION OF A STABLE FOLDING INTERMEDIATE WHICH CAN BE RESCUED BY THE CHAPERONINS GROEL AND GROES

Authors
PERALTA D HARTMAN DJ HOOGENRAAD NJ HOJ PB
Citation
D. Peralta et al., GENERATION OF A STABLE FOLDING INTERMEDIATE WHICH CAN BE RESCUED BY THE CHAPERONINS GROEL AND GROES, FEBS letters, 339(1-2), 1994, pp. 45-49
Citations number
28
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
339
Issue
1-2
Year of publication
1994
Pages
45 - 49
Database
ISI
SICI code
0014-5793(1994)339:1-2<45:GOASFI>2.0.ZU;2-2
Abstract
Pig heart mitochondrial malate dehydrogenase was chemically denatured in guanidine HC1. Upon 50-fold dilution of the denaturant spontaneous refolding could be observed in the temperature range 12-32 degrees C. At 36 degrees C spontaneous refolding was not observed but a stable fo lding intermediate that is fairly resistant to aggregation was formed. This intermediate is readily refolded by the chaperonins GroEL and Gr oES and may prove useful in future attempts to describe several aspect s of chaperonin action at physiological temperatures.