NA-DEPENDENT HIGH-AFFINITY UPTAKE OF L-GLUTAMATE IS PRIMARY CULTURES OF HUMAN FIBROBLASTS ISOLATED FROM 3 DIFFERENT TYPES OF TISSUE()

Cultured human fibroblasts isolated from embryonic muscle, skin and pe ripheral nerve tissues were found to accumulate [H-3]L-glutamate by a Na+-dependent uptake process strongly inhibited by several glutamate/a spartate analogues including D- and L-aspartate, D- and L-thleo-3-hydr oxyaspartate and L-trans-pyrrolidine-2,4-dicarboxylate but not D-gluta mate. It was also reduced by elevated concentrations of K+, Rb+ and Cs +. The values of K-m's were 5-20 mu M, well within the 'high affinity' region. Variations in the capacity (V-max) of [H-3]L-glutamate uptake did not correlate with the origin (muscle, skin or nerve tissue) of t he fibroblasts. The uptake characteristics suggest that it is mediated by a transport system similar to that commonly observed only in brain tissue.