T. Keller et al., A MONOCLONAL-ANTIBODY THAT INTERFERES WITH THE POST-AGGREGATION ADHESION OF DICTYOSTELIUM-DISCOIDEUM CELLS, FEBS letters, 339(1-2), 1994, pp. 119-123
A monoclonal antibody that interferes with the EDTA-resistant adhesion
of Dictyostelium discoideum slug cells recognised a carbohydrate epit
ope on four major antigens (95, 90, 35 and 30 kDa) in slug cells. The
35 and 30 kDa antigens were specific for stalks and spores, respective
ly. The 30 kDa antigen was identified as the cell surface glycoprotein
, PsA. Cyclic AMP, acting via cell surface receptors, induced only the
90 kDa slug cell antigen. Slug cell adhesion proteins may be involved
in cell-sorting and the glycosylation of the 95 and 90 kDa antigens a
ppeared to be abnormal in a mutant defective in cell-sorting. Previous
ly, a 150 kDa glycoprotein has been strongly implicated in slug cell a
dhesion and the present work suggests that additional glycoprotein(s)
are involved.