PORCINE OVARIAN GRANULOSA-CELLS SECRETE INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-4 AND PROTEIN-5 AND EXPRESS THEIR MESSENGER RIBONUCLEIC-ACIDS - REGULATION BY FOLLICLE-STIMULATING-HORMONE AND INSULIN-LIKE GROWTH FACTOR-I

Citation
Rw. Grimes et al., PORCINE OVARIAN GRANULOSA-CELLS SECRETE INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-4 AND PROTEIN-5 AND EXPRESS THEIR MESSENGER RIBONUCLEIC-ACIDS - REGULATION BY FOLLICLE-STIMULATING-HORMONE AND INSULIN-LIKE GROWTH FACTOR-I, Biology of reproduction, 50(3), 1994, pp. 695-701
Citations number
36
Categorie Soggetti
Reproductive Biology
Journal title
ISSN journal
00063363
Volume
50
Issue
3
Year of publication
1994
Pages
695 - 701
Database
ISI
SICI code
0006-3363(1994)50:3<695:POGSIG>2.0.ZU;2-G
Abstract
Using ligand blotting, Western immunoblotting, and Northern analysis, we have characterized the insulin-like growth factor (IGF)-binding pro teins (IGFBPs) produced by cultures of porcine granulosa cells. Ligand blot analysis of conditioned medium from untreated cultures of modera tely differentiated granulosa cells (MDGCs; from 4-6-mm follicles) rev ealed mainly IGF-binding activity associated with IGFBP-2 (34 kDa) and IGFBP-3 (40/44-kDa doubler), which have previously been identified an d characterized. In addition, these cultures secreted 30- and 22-kDa f orms under some circumstances. The identification and regulation of th ese IGFBPs of lower molecular mass were the focus of the current studi es. Treatment of these MDGCs with IGF-I dramatically stimulated the pr oduction (to a detectable level) of a 30-kDa IGFBP that was identified by immunoblotting with antiserum to IGFBP-5 but not antisera to IGFBP -1, -2, -3, -4, or -6. Production of IGFBP-5 was attenuated by concurr ent treatment with FSH. IGFBP-5 mRNA in these cultures was correspondi ngly stimulated by IGF-I but unaffected by FSH. FSH increased the leve l of a minor 22-kDa IGFBP. Messenger RNAs for IGFBP-1, -4, and -6 were also examined but only IGFBP-4 mRNA was detectable, suggesting that t he 22-kDa band was IGFBF-4. These results were compared to those in cu ltures of immature granulosa cells from 1-3.mm follicles, in which 22- and 30-kDa IGFBPs were readily detectable. An antiserum to IGFBP-4 pre cipitated the 22-and 30-kDa bands whereas deglycosylation shifted the 30-kDa IGFBP to 22 kDa, suggesting that both these bands represent gly cosylation variants of IGFBP-4. The present study demonstrated that IG FBPs of low molecular size are differentially expressed as a function of granulosa cell differentiation and are regulated by hormones and gr owth factors.