PORCINE OVARIAN GRANULOSA-CELLS SECRETE INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-4 AND PROTEIN-5 AND EXPRESS THEIR MESSENGER RIBONUCLEIC-ACIDS - REGULATION BY FOLLICLE-STIMULATING-HORMONE AND INSULIN-LIKE GROWTH FACTOR-I
Rw. Grimes et al., PORCINE OVARIAN GRANULOSA-CELLS SECRETE INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-4 AND PROTEIN-5 AND EXPRESS THEIR MESSENGER RIBONUCLEIC-ACIDS - REGULATION BY FOLLICLE-STIMULATING-HORMONE AND INSULIN-LIKE GROWTH FACTOR-I, Biology of reproduction, 50(3), 1994, pp. 695-701
Using ligand blotting, Western immunoblotting, and Northern analysis,
we have characterized the insulin-like growth factor (IGF)-binding pro
teins (IGFBPs) produced by cultures of porcine granulosa cells. Ligand
blot analysis of conditioned medium from untreated cultures of modera
tely differentiated granulosa cells (MDGCs; from 4-6-mm follicles) rev
ealed mainly IGF-binding activity associated with IGFBP-2 (34 kDa) and
IGFBP-3 (40/44-kDa doubler), which have previously been identified an
d characterized. In addition, these cultures secreted 30- and 22-kDa f
orms under some circumstances. The identification and regulation of th
ese IGFBPs of lower molecular mass were the focus of the current studi
es. Treatment of these MDGCs with IGF-I dramatically stimulated the pr
oduction (to a detectable level) of a 30-kDa IGFBP that was identified
by immunoblotting with antiserum to IGFBP-5 but not antisera to IGFBP
-1, -2, -3, -4, or -6. Production of IGFBP-5 was attenuated by concurr
ent treatment with FSH. IGFBP-5 mRNA in these cultures was correspondi
ngly stimulated by IGF-I but unaffected by FSH. FSH increased the leve
l of a minor 22-kDa IGFBP. Messenger RNAs for IGFBP-1, -4, and -6 were
also examined but only IGFBP-4 mRNA was detectable, suggesting that t
he 22-kDa band was IGFBF-4. These results were compared to those in cu
ltures of immature granulosa cells from 1-3.mm follicles, in which 22-
and 30-kDa IGFBPs were readily detectable. An antiserum to IGFBP-4 pre
cipitated the 22-and 30-kDa bands whereas deglycosylation shifted the
30-kDa IGFBP to 22 kDa, suggesting that both these bands represent gly
cosylation variants of IGFBP-4. The present study demonstrated that IG
FBPs of low molecular size are differentially expressed as a function
of granulosa cell differentiation and are regulated by hormones and gr
owth factors.