DNA STRETCHING ON FUNCTIONALIZED GOLD SURFACES

Authors
ZIMMERMANN RM COX EC
Citation
Rm. Zimmermann et Ec. Cox, DNA STRETCHING ON FUNCTIONALIZED GOLD SURFACES, Nucleic acids research, 22(3), 1994, pp. 492-497
Citations number
32
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
22
Issue
3
Year of publication
1994
Pages
492 - 497
Database
ISI
SICI code
0305-1048(1994)22:3<492:DSOFGS>2.0.ZU;2-T
Abstract
We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au - biotin - streptavidin biotin - DNA bonds. DNA anchore d to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at leas t 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.