Escherichia Coli (E. coli) was used as an index to assess the disinfec
tion of water-borne microorganisms by sunlight irradiation in the pres
ence of a titanium dioxide (TiO2, anatase) photocatelyst. With 23 min.
irradiation, more than 99% of the spiked E. Coli was inactivated in w
ater solutions. No significant changes in E. coli were observed in the
irradiated control with no TiO2 added, or in the water solution that
was not irradiated but contained TiO2. The inactivation of E. coli was
attributed to the generation of free hydroxyl radicals during irradia
tion of TiO2 in the aqueous solution.