REGENERATION OF DIHAPLOIDS VIA GYNOGENESIS IN ALLIUM-PORRUM L

Ovaries of three different genotypes of leek were cultivated on a MS b asic medium containing 1 ppm NAA, 8 ppm 2.iP, and sucrose of either 3 %, 6 %, or 3 % plus 1.6 % mannitol, respectively. Cultures were subjec ted to + 10-degrees-C for 0, 3, 6, or 12 days in darkness, followed by culture at + 25-degrees-C in a photo period of 16 hours. There was no consistent effect of low temperature treatment of explants or of cult ure media tested, but a genotype specific reaction with regard to rege neration of dihaploid plantlets via gynogenesis. Out of two genotypes under investigation only tetraploid, octoploid, and mixoploid plantlet s were obtained, whereas in a third genotype besides one tetraploid ei ght dihaploid clones could be raised, representing 0.16 % reacting ova ries. Three of the dihaploid lines show chlorophyll deficiencies.