IMPROVEMENT OF LATERAL RESOLUTION IN FAR-FIELD FLUORESCENCE LIGHT-MICROSCOPY BY USING 2-PHOTON EXCITATION WITH OFFSET BEAMS

Authors
HELL SW
Citation
Sw. Hell, IMPROVEMENT OF LATERAL RESOLUTION IN FAR-FIELD FLUORESCENCE LIGHT-MICROSCOPY BY USING 2-PHOTON EXCITATION WITH OFFSET BEAMS, Optics communications, 106(1-3), 1994, pp. 19-24
Citations number
9
Categorie Soggetti
Optics
Journal title
Optics communications → ACNP
ISSN journal
00304018
Volume
106
Issue
1-3
Year of publication
1994
Pages
19 - 24
Database
ISI
SICI code
0030-4018(1994)106:1-3<19:IOLRIF>2.0.ZU;2-K
Abstract
A method is described of increasing the resolution in far-field fluore scence light microscopy by a factor of 2. Calculations show that a lat eral resolution of 75 nm is achieved with a lens of numerical aperture of 1.4 (oil immersion) by a two-photon excitation mode with each phot on stemming from displaced focused light beams of lambda=500 nm and ov erlapping main maxima.