PH ON-OFF SWITCHING OF ANTIBODY HAPTEN BINDING BY SITE-SPECIFIC CHEMICAL MODIFICATION OF TYROSINE

Tetranitromethane (TNM) chemically mutates the binding sites of antibo dies so that the nitrated antibodies exhibit pa-dependent binding near physiological pH. Three monoclonal antibodies were selectively modifi ed, each under different conditions, with the resultant loss of bindin g activity at pH > 8 which is recovered at pH < 6. Recovery and loss o f binding are ascribed to the protonation and deprotonation, respectiv ely, of the hydroxyl group of the resulting 3-nitrotyrosine side chain (pK(a) similar to 7) at the binding site of these antibodies. pH on-o ff dependency of binding activity, common to all TNM-modified antibodi es studied by us so far, may find use in a variety of applications in which controlled modulation under mild conditions is required.