AUTOMATED MEASUREMENT OF ROOT LENGTH WITH A 3-DIMENSIONAL HIGH-RESOLUTION SCANNER AND IMAGE-ANALYSIS

Citation
Al. Smit et al., AUTOMATED MEASUREMENT OF ROOT LENGTH WITH A 3-DIMENSIONAL HIGH-RESOLUTION SCANNER AND IMAGE-ANALYSIS, Plant and soil, 158(1), 1994, pp. 145-149
Citations number
14
Categorie Soggetti
Agriculture Soil Science","Plant Sciences",Agriculture
Journal title
ISSN journal
0032079X
Volume
158
Issue
1
Year of publication
1994
Pages
145 - 149
Database
ISI
SICI code
0032-079X(1994)158:1<145:AMORLW>2.0.ZU;2-O
Abstract
For measuring the length of root samples, the use of a three-dimension al (3D) scanner is proposed to address the problem of a too low resolu tion. The scanner's high resolution (up to 354 pixels per cm) enables in the resulting grey-value image very thin roots (diameter 100 mu m) to be segmented from the background by a simple thresholding operation . After skeletonizing, total length of the roots is calculated by mult iplying the number of skeleton pixels by a correction factor. A compar ison with the modified Newman Line-Intersect Method showed a correlati on of r = 0.98. Besides its superior resolution, an advantage of this type of scanner is its focusing depth, which allows root samples to be recorded on the scanbed similarly to a camera-oriented system.