PERIPHERAL-BLOOD STEM-CELL COLLECTION USING THE SMALL-VOLUME COLLECTION CHAMBER IN THE FENWAL CS-3000 PLUS BLOOD-CELL SEPARATOR

Optimal methods for peripheral blood stem cell (PBSC) collection shoul d yield a small volume product containing minimal platelets and a larg e number of mononuclear cells (MNC). The Fenwal CS-3000 Plus blood cel l separator was modified in an attempt to meet these objectives, Modif ications of the CS-3000 Plus included use of the small volume collecti on chamber (SVCC), increasing the interface/offset detector setting to 150 and decreasing the centrifuge speed to 1400 rpm. Thirty-eight pat ients undergoing 224 PBSC collections were studied. Mobilization metho ds included 4 g/m(2) cyclophosphamide (CY), CY + 250 mu g/m(2) subcuta neous granulocyte-macrophage colony-stimulating factor (GM-CSF) or GM- CSF alone. The median collection volume was 58 ml containing a median of 21 ml of red blood cells. Platelet collection efficiency was < 4.4% and the median number of extracted platelets was 0.6 x 10(11)/apheres is. Median reduction in the platelet count post-apheresis was 15%. MNC purity was 95.5% and MNC collection efficiency was 61%. Yield of MNC was 1 x 10(8)/kg/apheresis. Collected progenitor cells correlated with both the WBC and MNC content of the apheresis product. The modified C S-3000 Plus with the SVCC is effective for PBSC collection following t hree different mobilization regimens and is, therefore, recommended fo r routine collection of PBSC.