H. Arai et al., REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR ANALYSIS OFURINARY PROTEINS - DIAGNOSTIC-SIGNIFICANCE OF ALPHA(1)-ACID GLYCOPROTEIN, Nephron, 66(3), 1994, pp. 278-284
In order to analyze urinary proteins from patients with various renal
diseases, a reversed-phase high-performance liquid chromatography with
IPG PACK ODS column packed with polyporous glass was employed. The pe
ak ai eas of alpha(1)-acid glycoprotein (alpha(1)-AGP), beta(2)-microg
lobulin (beta(2)-MG) and albumin were measured by a chromato-integrato
r. The alpha(1)-AGP/albumin ratio was regarded as the marker of glomer
ular damage, while the beta(2)-MG/albumin ratio indicated tubular dysf
unction. As a result, the alpha(1)-AGP/albumin ratio in the urine from
patients with either various glomerulonephritis (GN) or idiopathic ne
phrotic syndrome was significantly higher than that from either patien
ts with postural proteinuria or healthy children. However, the beta(2)
-MG/albumin ratio in the urine from patients with GN was the same leve
l as controls. The beta(2)-MG/albumin ratio was elevated only in urine
from patients with tubular dysfunctions. These data suggest that the
urinary alpha(1)-AGP/albumin ratio could be a beneficial indicator in
locating patients with GN from among children with asymptomatic protei
nuria