REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR ANALYSIS OFURINARY PROTEINS - DIAGNOSTIC-SIGNIFICANCE OF ALPHA(1)-ACID GLYCOPROTEIN

In order to analyze urinary proteins from patients with various renal diseases, a reversed-phase high-performance liquid chromatography with IPG PACK ODS column packed with polyporous glass was employed. The pe ak ai eas of alpha(1)-acid glycoprotein (alpha(1)-AGP), beta(2)-microg lobulin (beta(2)-MG) and albumin were measured by a chromato-integrato r. The alpha(1)-AGP/albumin ratio was regarded as the marker of glomer ular damage, while the beta(2)-MG/albumin ratio indicated tubular dysf unction. As a result, the alpha(1)-AGP/albumin ratio in the urine from patients with either various glomerulonephritis (GN) or idiopathic ne phrotic syndrome was significantly higher than that from either patien ts with postural proteinuria or healthy children. However, the beta(2) -MG/albumin ratio in the urine from patients with GN was the same leve l as controls. The beta(2)-MG/albumin ratio was elevated only in urine from patients with tubular dysfunctions. These data suggest that the urinary alpha(1)-AGP/albumin ratio could be a beneficial indicator in locating patients with GN from among children with asymptomatic protei nuria