EFFECTS OF PSEUDOSTELLARIA-HETEROPHYLLA ON PROLIFERATION AND DIFFERENTIATION OF MURINE BONE-MARROW CELLS

The incorporation of tritiated thymidine into the DNA of murine bone m arrow cells could be stimulated by fraction PH-I Ba separated from the roofs of Pseudostellaria heterophylla in a dose-dependent manner ill vitro. Moreover, PH-I Ba could induce the differentiation of murine bo ne marrow cells from pluripotent haemopoietic stem cells into macropha ges-like cells in vitro. Autocrine or paracrine stimulation of granulo cyte-macrophage colony stimulating factor was likely to underly the in duction of differentiation. Therefore, PH-I Ba was proved to be an imm unostimulating agent for mouse marrow hematopoiesis and was found to b e a polysaccharide. The sugar components were analyzed by Gas Liquid C hromatography of their alditol acetates.