S. Pritlovecarson et al., IMMUNOHISTOCHEMICAL ANALYSIS OF TISSUES REGENERATED FROM WITHIN PERIODONTAL DEFECTS TREATED WITH EXPANDED POLYTETRAFLUOROETHYLENE MEMBRANES, Journal of periodontology, 65(2), 1994, pp. 134-138
IMMUNOCYTOCHEMICAL ANALYSIS WAS CARRIED out on samples of 5-, 6-, and
9-week old regenerated soft tissue taken from healing periodontal defe
cts treated by guided tissue regeneration using expanded polytetrafluo
rethylene (ePTFE) membranes. A panel of monoclonal and polyconal antib
odies to cytokeratins, vimentin, and collagen was used to label cells
and collagen types I, III, and IV. Epithelium was identified in 7 of t
he 9 samples examined, in addition to mesenchymal cells staining posit
ively for vimentin and co-distribution of collagen types I, III, and I
V in all samples. Clinical observations indicated that exposure of the
ePTFE membranes during healing was a frequent occurrence, and the pre
sence and quantity of epithelium found within the healing defect benea
th the membrane may be related to the extent to which this occurs.