DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES

Citation
C. Delcasino et al., DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES, Biology of the cell, 79(2), 1993, pp. 125-132
Citations number
26
Categorie Soggetti
Cytology & Histology
Journal title
ISSN journal
02484900
Volume
79
Issue
2
Year of publication
1993
Pages
125 - 132
Database
ISI
SICI code
0248-4900(1993)79:2<125:DOMDTG>2.0.ZU;2-U
Abstract
The distribution of microtubules was investigated in Nicotiana tabacum pollen tubes at different stages of tube growth by immunofluorescence microscopy. Using specific antibodies, the presence of microtubules c onsisting of different tubulin isoforms was tested. alpha-, beta- and tyrosinated alpha-tubulin were present within the tube, whereas the ac etylated form was lacking. The presence of tubulin subunits in pollen tube extracts was also investigated by immunoblotting analyses. The us e of a confocal laser scanning microscope integrated with computer-ass isted imaging, allowed a detailed visualization of the microtubule dis tribution and organization. Cytoplasmic microtubules organized as shor t bundles with various orientations were detected at the apex of long tubes.