DEMONSTRATION OF AMYLOID BETA-PROTEIN SECRETION IN A MOUSE NEURONAL CELL-LINE

We investigated the secretion of amyloid beta-protein (beta AP) in a m ouse neuronal cell line SN49. SN49 cells stably transfected with mouse beta-amyloid precursor protein (APP) 695 cDNA released approximately three times greater amounts of a 4 kDa protein immunoreactive with ant i-beta AP antibodies than untransfected and mock-transfected cells. Th is 4 kDa protein was further identified as mouse PAP by direct amino a cid sequence analysis. These results strongly suggest that the beta AP secretion occurs in mouse neuronal cells as in human cells.