SYNTHESIS IN SOLUTION OF OLIGODEOXYNUCLEOTIDES AND SOME THEIR 5'-LINKED AND 3'-LINKED DERIVATIVES

Antisense oligodeoynucleotides (ODNs) and their derivatives are highly interesting fools to regulate gene expression and promising drugs for antiviral and anticancer therapy. In view of performing more extensiv e pharmacological trials requiring relatively great amounts of ODNs, w e used a method of ODN synthesis derived from the phosphotriester appr oach which allow to prepare ODNs covalently linking cholesteryl residu e (in 5' or 3') and phenazinium group (in 5'). HPLC purifications are discussed.